| EPSRC Reference: |
GR/S85467/01 |
| Title: |
Pilot study for in-vivo two-photon flourescence imaging of sensory-evoked responses in the mouse visual cortex. |
| Principal Investigator: |
Schultz, Professor SR |
| Other Investigators: |
|
| Researcher Co-Investigators: |
|
| Project Partners: |
|
| Department: |
Physiology |
| Organisation: |
UCL |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
15 February 2004 |
Ends: |
14 August 2004 |
Value (£): |
21,781
|
| EPSRC Research Topic Classifications: |
| Biomedical neuroscience |
Vision & Senses - ICT appl. |
|
| EPSRC Industrial Sector Classifications: |
| Creative Industries |
Information Technologies |
|
| Related Grants: |
|
| Panel History: |
|
|
Summary on Grant Application Form |
|
Much of what we have learned about how the brain processes sensory information has come from micro-electrode recordings of the outputs of cortical neurons. To go further, and discriminate between different models of how computation is being performed in those cells, however, we need new technology capable of examining neuronal inputs as well as outputs while a sensory stimulus is presented to a live animal. The research we propose here involves refining a technique which is capable of just that: fluorescence imaging of calcium transient signals in nerve cells can be performed with a spatial scale at the level of dendritic spines (microns), and a time resolution as low as milliseconds. By imaging calcium signals in the dendritic trees of neurons in the primary visual cortex of the mouse while a visual stimulus is presented on a video monitor, we will record signals which reflect the inputs to a neuron at the same time as recording the output of the neuron electrophysiologically. By imaging at a larger spatial scale, we will also be able to record the activity of many neurons simultaneously. An in-vivo two- photon microscope facility at the Wolfson Institute for Biomedical Research will be augmented to enable visual physiology studies to be performed on it, and pilot imaging experiments carried out. The expected outcome of the project is a demonstration of the use of the technique for studying visual information processing, and an assessment of its suitability for addressing outstanding problems in visual neuroscience
|
|
Key Findings |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Potential use in non-academic contexts |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Impacts |
|
| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
|
| Date Materialised |
|
|
|
|
Sectors submitted by the Researcher |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
| Project URL: |
|
| Further Information: |
|
| Organisation Website: |
|