| EPSRC Reference: |
GR/S76113/01 |
| Title: |
Surface Derivitization of Conducting Polymer with Immunospecific Biomolecules for Impedance Spectroscopy-Based Biosensors |
| Principal Investigator: |
Vadgama, Professor P |
| Other Investigators: |
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| Department: |
Biomedical Materials |
| Organisation: |
Queen Mary University of London |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
19 March 2004 |
Ends: |
18 March 2005 |
Value (£): |
11,002
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| EPSRC Research Topic Classifications: |
| Chemical Biology |
Materials Processing |
| Medical science & disease |
Surfaces & Interfaces |
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| EPSRC Industrial Sector Classifications: |
| Electronics |
Pharmaceuticals and Biotechnology |
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Summary on Grant Application Form |
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A study of the impedance properties of conducting polymer films applied over gold electrodes at the host institution has indicated that the magnitude and phase shift of the film's voltage/current response over a wide frequency range may be useful for detecting the presence of specific molecules in liquids at the film/liquid interface. Application of this sensitive detection technique to biosensors, but by specific derivatizing of the surface of the polymer film with affinity biomolecules will also specifically bind with biomolecules of interest to provide a linear impedance response correlated with analyte concentration. The overall goal of this project is to functionalize the surface of a thin film of a conducting polymer, notably poly- (3methylthiophene) and polypyrrole, with a uniform coverage of an enzyme or antibody with highly specific binding affinity for target biomolecules. The impedance spectroscopic characteristics of such a film would then be determined both in the presence and absence of the target biomolecule to establish the magnitude of the change in response to analyte. Comparison of the results from surface-functionalized films with the previously-studied polymer-entrapped enzyme/antibody film will provide valuable insights into how the reversible target-receptor molecules binding effects conductivity of the polymer. Results of these studies will also support development of biosensors for a wide range of target biomolecules. The organic chemistry experience of the visiting Fellow will help extend the work on impedimetric biosensors.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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