| EPSRC Reference: |
EP/D000696/1 |
| Title: |
The effects of fluid flow on protein structure |
| Principal Investigator: |
Blanch, Professor EW |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Life Sciences |
| Organisation: |
University of Manchester, The |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 January 2006 |
Ends: |
31 December 2008 |
Value (£): |
31,742
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| EPSRC Research Topic Classifications: |
| Analytical Science |
Multiphase Flow |
| Reactor Engineering |
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| EPSRC Industrial Sector Classifications: |
| No relevance to Underpinning Sectors |
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| Panel History: |
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Summary on Grant Application Form |
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A variety of physical and chemical perturbations are routinely used to probe protein structure. Mechanical perturbations have also been used and are particularly informative as they can allow the forces required to modify protein structure and function to be accurately quantified. We will explore and develop a new mechanical perturbation, fluid-flow turbulence, and assess its applicability for studying, and controlling, protein unfolding.Turbulent flows will be deliberately induced in purpose-built flow cells to cause unfolding in several test proteins and so identify suitable targets for further study. Protein unfolding will be characterised using Raman spectroscopy and Raman optical activity, as these are proven and informative techniques. The observed conformational changes will be correlated with the measure flow parameters to further refine flow cell design. From the new information obtained in this pioneering project we hope to assess the viability of this approach for future, and more focused, studies of controlling protein structure using flow characteristics.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.man.ac.uk |