| EPSRC Reference: |
GR/S98689/01 |
| Title: |
Copper and arsenic speciation in liver as a model system for the development of a novel generic approach to determining tissue metal-protein interacti |
| Principal Investigator: |
Feldmann, Professor J |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Chemistry |
| Organisation: |
University of Aberdeen |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 November 2004 |
Ends: |
31 January 2008 |
Value (£): |
225,760
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| EPSRC Research Topic Classifications: |
| Analytical Science |
Chemical Biology |
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| EPSRC Industrial Sector Classifications: |
| Chemicals |
Pharmaceuticals and Biotechnology |
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| Related Grants: |
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| Panel History: |
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Summary on Grant Application Form |
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Metals and metalloids are an integral part of an organism and occur as an important component of some biomolecules. Although essential, metals in high concentrations can also generate toxic effects. Since the requirement of cells for these metals are different and are often regulated by biomolecular mechanisms, the accumulation and uptake as well as the resulting toxic effect is highly dependent on the form in which the metal occurs in the organism. Hence, it is important to identify the different forms in which metals and metalloids occur in different tissues. Here, a generic multi-method approach using different types of separation techniques and several types of mass spectrometry customized to the identification and quantification of metal containing proteins will be developed. A three phase project is proposed utilising metal screening technique in the initial phase, which specifically targets the metal-containing proteins which will be selected for the development of further detailed identification and quantification methods. In phase three the metal screening and mapping method will be applied directly to tissue sections in order to identify zones of differential metal accumulation. The different zones will be investigated for their metal speciation and the observations will be linked to the pathological differences. The model tissue is the liver.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.abdn.ac.uk |