| EPSRC Reference: |
GR/S78124/01 |
| Title: |
Chemical Analysis of the Mupirocin Gene Cluster from Pseudomonas fluorescens |
| Principal Investigator: |
Simpson, Professor TJ |
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| Project Partners: |
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| Department: |
Chemistry |
| Organisation: |
University of Bristol |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 October 2004 |
Ends: |
31 December 2007 |
Value (£): |
335,522
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
Bioprocess Engineering |
| Chemical Biology |
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| EPSRC Industrial Sector Classifications: |
| Pharmaceuticals and Biotechnology |
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Summary on Grant Application Form |
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Mupirocin (pseudomonic acid) is a structurally unusual polyketide antibiotic, which also displays a unique mode of action giving it potential for the treatment of multi-resistant bacteria. Due to its inherent instability in serum its use has been limited and so production of more stable active analogues is desirable. However its structural complexity makes realisation of this goal by traditional medicinal chemistry approaches problematic. Isolation and analysis of it biosynthetic gene cluster reveals a structure quite unlike any previously studied polyketide. Complete analysis of its gene cluster will require extensive chemical-based studies to complement on-going genetic manipulations. Combined chemical and genetic studies to achieve a full understanding of the details of mupirocin biosynthesis in Pseudomonas fluorescens could enable the production of analogues by rational manipulation of the biosynthetic genes to produce predictable alterations of structure. Very recent work has indicated that some other biologically interesting and evolutionary diverse systems may have generic similarities to mupirocin at the genetic level so this work will have wider implications for our ability to produce novel bioactive compounds by the combinatorial biosynthetic approach.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.bris.ac.uk |