| EPSRC Reference: |
GR/S67388/01 |
| Title: |
Single Biomolecule Dynamical Spectroscopy: Simulation and Experiment |
| Principal Investigator: |
McLeish, Professor T |
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| Department: |
Physics and Astronomy |
| Organisation: |
University of Leeds |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 July 2003 |
Ends: |
30 June 2004 |
Value (£): |
74,366
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| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Protein folding and unfolding urgently requires both new experimental and theoretical/simulation techniques that respect the high dimensionality of the folding space (or high number of degrees of freedom) to make progress. We propose a pilot project to explore novel mechanical unfolding/folding experiments made alongside interpretative simulations. Informed by existing single molecule mechanical unfolding at Leeds, and by the expertise in interpreting frequency-dependent linear rheology in terms of polymer structure, we will develop and apply an oscillatory spectroscopy to concatemers of proteins, as well as to model bioheteropolymers such as dextran. The amplitude and phase shift of the single-molecule compliance as a function of mean strain level and frequency will provide an unprecedentedly rich measure of the folding landscape. The simulations will run at both single and united-atom levels, and will concentrate on interpreting the large phase-shifts in the oscillatory signals expected when intermediate states are present on the unfoldign pathway, as well as guiding the choice of experiments. The experiment-simulation comparison will guide the development of a new highdimensional approach to protein-folding devised in the Leeds group.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.leeds.ac.uk |