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Details of Grant 

EPSRC Reference: GR/S64172/01
Title: Modified primers for highly multiplexed PCR on forensic samples
Principal Investigator: Brown, Professor T
Other Investigators:
Researcher Co-Investigators:
Project Partners:
Forensic Science Service Ltd
Department: Sch of Chemistry
Organisation: University of Southampton
Scheme: Standard Research (Pre-FEC)
Starts: 01 February 2004 Ends: 31 July 2007 Value (£): 207,143
EPSRC Research Topic Classifications:
Analytical Science Chemical Biology
Combinatorial Chemistry
EPSRC Industrial Sector Classifications:
Pharmaceuticals and Biotechnology Chemicals
Related Grants:
Panel History:  
Summary on Grant Application Form
The analysis of short tandem repeats (STRs) forms the basis of the current forensic DNA profiling system. This powerful technique was invented and developed in the UK, and is now used throughout the world. In this methodology several PCR reactions are carried out simultaneously in a single tube (multiplex PCR), often on very small and/or degraded samples of DNA isolated from scene of crime environments. The potential size of a multiplex is limited by the tendency of individual PCR primers to interact and form primer-dimers, which spoil the assay. We propose to develop novel chemically modified PCR primers to prevent these problems. This will allow very large multiplexes to be developed and greatly extend the reliability, accuracy and utility of DNA analysis in forensic science. The importance of multiplex PCR in the field of forensic science is immense. It extends from DNA profiling to the analysis of single nucleotide polymorphisms (SNPs). The aim of this project is to greatly increase the efficiency of multiplex PCR and to apply the improved methods to severalareas of forensic science.
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Organisation Website: http://www.soton.ac.uk