| EPSRC Reference: |
GR/S25159/01 |
| Title: |
Generic Methods to identify mechanisms of inactivation of biocatalysts during practical operation |
| Principal Investigator: |
Halling, Professor PJ |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Pure and Applied Chemistry |
| Organisation: |
University of Strathclyde |
| Scheme: |
Faraday (PreFEC) |
| Starts: |
18 June 2003 |
Ends: |
17 July 2006 |
Value (£): |
317,917
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| EPSRC Research Topic Classifications: |
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| EPSRC Industrial Sector Classifications: |
| Chemicals |
Pharmaceuticals and Biotechnology |
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Summary on Grant Application Form |
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Biocatalysts normally become inactivated during use, but the reason is usually unknown. We aim to develop methods to determine the cause of activ loss, as a basis for rational plans to improve stability. The methods will cover: 1) high yield recovery of adsorbed proteins from biocatalysts without (further) covalent modification: 2) identification of covalent changes in recovered enzyme molecules, using the approaches of proteomics (electrophoresis, mass spectroscopy and chromatography), but developing new interpretation strategies appropriate to modifcations found with biocatalysts; 3) detection and characterisation of non-covalent conformational changes in proteins in situ in biocatalyst particles by appropriate physic techniques (fluorescence, infra-red and circular dichroism) and protease susceptibility; 4) recovery of peptides for analysis from covalently attached proteins, using defined and limited cleavage. This is a flagship project of the Pro-Bio Faraday Partnership.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.strath.ac.uk |