| EPSRC Reference: |
GR/S05342/01 |
| Title: |
STIMULUS RESPONSIVE POLYMERIC PARTICLES FOR DELIVERY OF CELLS FOR TISSUE ENGINEERING |
| Principal Investigator: |
MacNeil, Professor S |
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| Department: |
Materials Science and Engineering |
| Organisation: |
University of Sheffield |
| Scheme: |
Postdoctoral Mobility PreFEC |
| Starts: |
01 July 2002 |
Ends: |
30 June 2003 |
Value (£): |
75,483
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Summary on Grant Application Form |
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Clinical evidence shows that it is possible to achieve wound healing for patients with chronic non-healing venous leg ulcers and diabetic foot ulcers if sufficient repeated applications of cells are used. However, there exist problems in developing a clinically effective cell delivery system. We propose to develop a new culture strategy by using novel polymeric particles for delivering to the wound bed autologous keratinocytes capable of initiating rapid wound healing. The polymeric particles will have a core-shell structure. The outer layer will be formed from a thermoresponsive polymer and presents a hydrophobic outer surface at the temperature for cell culture, allowing cell adhesion and proliferation. On application of the cell-cultured particles to the wound bed, the temperature will be deliberately lowered, and thus the thermoresponsive macromolecules will hydrate, leading to rapid swelling of the outer layer. The thermal modulated change of the outer surface from hydrophobic to hydrophilic will initiate cell detachment. Assessment of the performance of cells delivered from the particles will be undertaken using two in vitro wound bed models, one an ECM monolayer and the other a reconstructed human skin model. The latter provides many of the features of normal skin and will be ideal for assessing the impact of changes in temperature on the delivery of cells from particles looking at the ability of the delivered cells to form a normal epithelium in vitro.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.shef.ac.uk |