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Details of Grant 

EPSRC Reference: GR/R81916/01
Title: Second harmonic imaging of cell membrane structures
Principal Investigator: O'Shea, Professor P
Other Investigators:
Morgan, Prof. S Somekh, Professor M
Researcher Co-Investigators:
Project Partners:
Department: Sch of Biomedical Sciences
Organisation: University of Nottingham
Scheme: Postdoctoral Mobility PreFEC
Starts: 01 March 2002 Ends: 28 February 2003 Value (£): 70,414
EPSRC Research Topic Classifications:
Cells Chemical Biology
Complex fluids & soft solids Surfaces & Interfaces
EPSRC Industrial Sector Classifications:
No relevance to Underpinning Sectors
Related Grants:
Panel History:  
Summary on Grant Application Form
The purpose of this work is to allow the named postdoctoral Research Assistant, NBES, to transfer knowledge and technology between the Optical Engineering Group (OEG) of the School of Electrical and Electronic Engineering and the Cell Biophysics Group (CBG) of the School of Biomedical Sciences under the Postdoctoral mobility scheme, both schools being parts of the University of Nottingham.The project will demonstrate the feasibility of imaging cell membranes using the novel second harmonic imaging modes of TIR, SP and SP with layered media. They will be implemented by reconfiguring an existing short pulse, multi-photon excitation confocal microscope. The results obtained with these modes will be compared with those obtained using established methods. This will include images captured using a wide field confocal microscope developed by NBES in the OEG. The research will establish the difference in the structural and functional information provided by each of the novel and established modes. A model relating the response of each imaging mode to the susceptibilities of a sample will be initiated.The binding of macromolecules such as Alzheimers peptides and serum albumin with artificial membrane systems has been chosen as a subject for the various imaging modes as we have already shown that they exhibit substantial structural changes on binding to membranes.
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Further Information:  
Organisation Website: http://www.nottingham.ac.uk