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Details of Grant 

EPSRC Reference: GR/R36503/01
Title: The mechanism of twin-argenine signal peptide sequences for translocating proteins in the folded state.
Principal Investigator: Robinson, Professor C
Other Investigators:
Rodger, Dr R Rodger, Professor PM
Researcher Co-Investigators:
Project Partners:
Department: Biological Sciences
Organisation: University of Warwick
Scheme: Standard Research (Pre-FEC)
Starts: 11 February 2002 Ends: 10 August 2003 Value (£): 76,530
EPSRC Research Topic Classifications:
Biological membranes Chemical Biology
Protein chemistry Protein folding / misfolding
EPSRC Industrial Sector Classifications:
Pharmaceuticals and Biotechnology
Related Grants:
Panel History:  
Summary on Grant Application Form
This proposal is for a joint theoretical/experimental study of the mechanisms by which twin-argenine signal peptide sequences specifically direct the translocation of folded proteins through a range of biological membranes. A combination of atomistic computer simulation and biophysical spectyroscopic studies on synthetic signal peptides will be used to identiy: i) the secondary structure of the signal sequence ii) how this depends on environment (aqueous membrane-boung or intramembrance) and iii) the sequence of events surrounding insertion into the membrane. The detailed molecular-level information available from the simulations, rigorously validated against structgural information obtained from the experiments, will provide a much deeper understanding of this transaction mechanism than has previously been available.The project represents a new collaborative venture between two established research groups in Biological Sciences and Chemistry at Warwick, and will involve the transfer of a current EPSRC PDRA (Dr M A San Miguel) from Chemistry to Biological Sciences. While working with PMR, MASM has developed expertise in using molecular modelling to understand complex interfaces in materials chemistry. This project will allow him both to tansfer this expertise to biological applications and to broaden his own background. It will also be an excellent opprtunity for the applicants to explore the potential for initiating a much wider-ranging collaboration.
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Further Information:  
Organisation Website: http://www.warwick.ac.uk