| EPSRC Reference: |
GR/N21352/01 |
| Title: |
BIOSYNTHESIS OF HIGHLY REDUCED FUNGAL POLYKETIDE METABOLITES; ISOLATION OF FUNGAL PKS GENES |
| Principal Investigator: |
Simpson, Professor TJ |
| Other Investigators: |
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| Project Partners: |
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| Department: |
Chemistry |
| Organisation: |
University of Bristol |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 March 2001 |
Ends: |
30 November 2004 |
Value (£): |
258,438
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
Cells |
| Genomics |
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| EPSRC Industrial Sector Classifications: |
| Chemicals |
Pharmaceuticals and Biotechnology |
| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Fungal polyketide metabolites are a major potential source of novel bioactive compounds. Recent advances in bacterial polyketide biosynthesis have realised the goal of producing new compounds by rational manipulation of the biosynthetic pathway at the genetic level. In contrast, similar work on fungal polyketides, e.g. squalestatins and decarestrictines, will be isolated by synthesis of the potential advanced intermediates for biosyntheticstudies to elucidate the details of the assembly pathway and as the basis of a bioassay to allow the isolation and purification of the PKS enzymes for a reverse-genetics approach to isolate the PKS genes. A wide variety of modern synthetic methodology will be applied to the synthesis of the structurally complex intermediates. Assay procedures will make use of sensitive HPLC and HPLC/MS techniques. Partial amino acid sequence information from the purified enzymes will be used to design oligonucleotide probes to isolate the PKS for full sequencing.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.bris.ac.uk |