| EPSRC Reference: |
GR/N19052/01 |
| Title: |
MULTIPHOTON,X-RAY&UV MICRO-IRRADIATION OF SUB-CELLULAR COMPONENTS TO TRACK INTRA-CELLULAR DYNAMICS |
| Principal Investigator: |
Wharton, Professor C |
| Other Investigators: |
|
| Researcher Co-Investigators: |
|
| Project Partners: |
|
| Department: |
Sch of Biosciences |
| Organisation: |
University of Birmingham |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 April 2000 |
Ends: |
30 September 2002 |
Value (£): |
144,137
|
| EPSRC Research Topic Classifications: |
|
| EPSRC Industrial Sector Classifications: |
| Pharmaceuticals and Biotechnology |
No relevance to Underpinning Sectors |
|
| Related Grants: |
|
| Panel History: |
|
|
Summary on Grant Application Form |
|
The central aim in modern biochemistry is to observe, as directly as possible, enzymatic processes in living cells. To achieve this, methods need to be developed for initiating reactions in very small subcellular volumes. Techniques of deep UV and multiphoton micro-irradation which will allow discrete areas of a cell nucleus to be irradiated offer a unique opportunity to study the dynamic responses of cell proteins to the induction of DNA damage. Once this is achieved the dynamic response, in terms of position, rate, topology and kinetics of DNA and nuclear proteins to UV-induced damage can be studied.Development of precision micro-masking and spectral tailoring of soft x-rays from a pulsed laser plasma source will allow the dynamics of intercellular communication between damaged and undamaged cells to be observed.This multidisciplinary project, at the boundary between the physical and life sciences, will provide state-of-the-art technology to underpin fundamental research in the areas of biochemistry and biological cell dynamics.
|
|
Key Findings |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Potential use in non-academic contexts |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Impacts |
|
| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
|
| Date Materialised |
|
|
|
|
Sectors submitted by the Researcher |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
| Project URL: |
|
| Further Information: |
|
| Organisation Website: |
http://www.bham.ac.uk |