| EPSRC Reference: |
GR/N10950/01 |
| Title: |
ASYMETRIC CHARGE NEUTRALISATION IN PROTEIN INDUCED DNA-BENDING |
| Principal Investigator: |
Allemann, Professor RK |
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| Department: |
School of Chemistry |
| Organisation: |
University of Birmingham |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
03 July 2000 |
Ends: |
02 September 2003 |
Value (£): |
62,941
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
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| EPSRC Industrial Sector Classifications: |
| Pharmaceuticals and Biotechnology |
No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Non-covalent interactions between nucleic acids and proteins govern biological process such as replication, recombination, and transcription. The formation of protein-DNA complexes are often accompanied by significant conformational changes of both reaction partners in order to ensure an optimal fit. While there is increasing evidence suggesting that assymmetic charge neutralisation is one of the mechanisms used by proteins to induce DNA bending, little is known about the thermodynamic parameters which control such association reactions. In order to determine the contribution of charge neutralisation to the overall stability of the DNA complexes of MEF-2C, the free energy of complex formation will be determined by electrophoretic mobility shift assays for complexes in which individual charge interactions are removed by replacing either negatively charged phosphodiester linkages with uncharged methylphosphonates or positively charged residues of MEF-2C with uncharged ones. In addition to providing information about the thermodynamics of charge neutralisation, such an approach should also allow us to measure the free energy required to bend the free DNA into the conformation which it adopts in the MEF-2C complex, thereby providing a thermodynamic bendability scale of the oligonucleotides.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.bham.ac.uk |