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EPSRC Reference: GR/M89553/01
Title: CHARACTERISATION OF PASSIVE LEACHATE TREATMENT SYSTEMS BY QUANTITIVE PCR ANALYSIS
Principal Investigator: Moffett, Dr B
Other Investigators:
Harris, Professor J
Researcher Co-Investigators:
Project Partners:
Cleanaway Ltd
Department: Health, Sport and Biosciences
Organisation: University of East London
Scheme: Standard Research (Pre-FEC)
Starts: 01 November 1999 Ends: 31 October 2002 Value (£): 227,209
EPSRC Research Topic Classifications:
Assess/Remediate Contamination
EPSRC Industrial Sector Classifications:
No relevance to Underpinning Sectors Environment
Related Grants:
Panel History:  
Summary on Grant Application Form
Active pollution control systems at landfill sites are expensive to operate. In order to operate passive leachate treatment systems the microbial communities responsible for the treatment must be characterised in terms of composition and population dynamics. Using the Pitsea site as a model we will characterise quantitatively the microbial communities responsible for the treatment within the perimeter ditch. This will be achieved by the molecular analysis of 16S rDNA extracted from the samples and so will identify both culturable and non-culturable bacteria. Identification of a portion of the organisms present will be by reference to the available databases. Quantification of known and unknown groups will be achieved using real-time quantitative PCR analysis which requires a high specification thermal cycler able to monitor the accumulation of amplification products as the reaction proceeds. The information gained will enable the efficiency of passive treatment systems within the perimeter ditches at Pitsea and similar landfill sites to be increased by appropriate management regimes. The probes that will be developed are likely to find a number of applications in the landfill and water treatment industries.
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Organisation Website: http://www.uel.ac.uk