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Details of Grant 

EPSRC Reference: GR/M70797/01
Title: FLUORESCENCE MICROSCOPY AND COMBINATORIAL CHEMISTRY - PROXIMITY SCREENING AND SENSOR ARRAYS
Principal Investigator: Bradley, Professor M
Other Investigators:
Brocklesby, Dr WS Frey, Professor JG
Researcher Co-Investigators:
Project Partners:
Department: School of Chemistry
Organisation: University of Southampton
Scheme: Standard Research (Pre-FEC)
Starts: 01 October 1999 Ends: 30 September 2001 Value (£): 218,257
EPSRC Research Topic Classifications:
Combinatorial Chemistry
EPSRC Industrial Sector Classifications:
No relevance to Underpinning Sectors
Related Grants:
Panel History:  
Summary on Grant Application Form
Fluorescence is becoming the major analytical tool in screening. It is very sensitive, environmentally friendly, robust and applicable to virtually all screening formats. Solid phase resins are an ideal method of presenting fluorophores for interrogation. They allow a high concentration of fluorophore to be presented, are reusable, allow rapid microscopic examination and require only tiny amounts of analyte for analysis. In addition the advent of combinatorial chemistry and the one bead one compound approach means that new fluorescent based sensors can be both rapidly prepared and screened.The first aim of the proposal will be to obtain a fundamental understanding of the nature of resin beads in association with fluorescent probes in such a manner that resin based fluorophores can be used for sensing and screening experiments.Having determined the physical and optical requirements for optimal bead screening and interrogation the beads will be used in a number of assays. Firstly in the area of pH, Ca2+ and 02 sensing, secondly fluorophores on beads will be used for proximity screening using an energy transfer process and thirdly fluorescence will be used for the determination of association constants between ligands on beads and proteins or host molecules.
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Organisation Website: http://www.soton.ac.uk