| EPSRC Reference: |
GR/M70797/01 |
| Title: |
FLUORESCENCE MICROSCOPY AND COMBINATORIAL CHEMISTRY - PROXIMITY SCREENING AND SENSOR ARRAYS |
| Principal Investigator: |
Bradley, Professor M |
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| Department: |
Sch of Chemistry |
| Organisation: |
University of Southampton |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 October 1999 |
Ends: |
30 September 2001 |
Value (£): |
218,257
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| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Fluorescence is becoming the major analytical tool in screening. It is very sensitive, environmentally friendly, robust and applicable to virtually all screening formats. Solid phase resins are an ideal method of presenting fluorophores for interrogation. They allow a high concentration of fluorophore to be presented, are reusable, allow rapid microscopic examination and require only tiny amounts of analyte for analysis. In addition the advent of combinatorial chemistry and the one bead one compound approach means that new fluorescent based sensors can be both rapidly prepared and screened.The first aim of the proposal will be to obtain a fundamental understanding of the nature of resin beads in association with fluorescent probes in such a manner that resin based fluorophores can be used for sensing and screening experiments.Having determined the physical and optical requirements for optimal bead screening and interrogation the beads will be used in a number of assays. Firstly in the area of pH, Ca2+ and 02 sensing, secondly fluorophores on beads will be used for proximity screening using an energy transfer process and thirdly fluorescence will be used for the determination of association constants between ligands on beads and proteins or host molecules.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.soton.ac.uk |