Details of Grant 

EPSRC Reference:	GR/M44477/01
Title:	NUCLEOSIDE ANALOGUES FOR STUDYING DNA RECOGNITION & REPAIR BY AN ALKYLTRANSFERASE
Principal Investigator:	Williams, Dr D
Other Investigators:
Researcher Co-Investigators:
Project Partners:
Department:	Chemistry
Organisation:	University of Sheffield
Scheme:	Standard Research (Pre-FEC)
Starts:	01 June 1999	Ends:	31 May 2000	Value (£):	50,531
EPSRC Research Topic Classifications:	Biological & Medicinal Chem.
EPSRC Industrial Sector Classifications:	No relevance to Underpinning Sectors
Related Grants:
Panel History:
Summary on Grant Application Form
06-Alkylguanine-DNA alkyltransferase (Atase) is found in many organisms and repairs the highly promutagenic lesion 06-methylguanine in DNA. It is also an important target in chemotherapeutic regimes used in cancer treatment. Despite a high resolution crystal structure of the E.coli Atase (Ada protein), the exact processes of recognition and repair by these important proteins remains largely unknown. In the proposed study, we will prepare oligonucleotides containing nucleoside analogues for investigating the mechanism of action of the mammalian ATase. We will also investigate the nucleoside analogues as inhibitors for ATase. It is anticipated that the modified oligonucleotides will be valuable for obtaining covalent complexes with ATase for use in structural analysis by crystallography.
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Organisation Website:	http://www.shef.ac.uk