| EPSRC Reference: |
GR/M13213/01 |
| Title: |
PROTEIN SEPARATIONS USING 2-DIMENSIONAL CAPILLARY ELECTROPHORESIS |
| Principal Investigator: |
Manz, Prof Dr A |
| Other Investigators: |
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| Department: |
Chemistry |
| Organisation: |
Imperial College London |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 January 1999 |
Ends: |
31 December 2000 |
Value (£): |
106,988
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| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Protein separations are achieved today using 2d slab gel electrophoresis and silver staining. This method is offering peak capacities of more than 10,000. However, reproducibility and speed are unsatisfactory.The proposed work shall develop microchip technology for miniaturisations in capillaries. Theory predicts faster separations with identical separation efficiencies for smaller volumes. Proteome separations should be achievable within 5 minutes.Glass, quartz and PDMS microstructures will be designed, manufactured and tested for their compatibility with isoelectric focusing (1st dimension) and gel electrophoresis (2nd dimension). In the closed capillaries, detection will be achieved by pre-column labelling with a flurophore or, alternatively, by detecting the native fluorescence of proteins (Trp).
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.imperial.ac.uk |