| EPSRC Reference: |
GR/L34273/01 |
| Title: |
HIGH RESOLUTION FLUORESCENCE LIFETIME IMAGING |
| Principal Investigator: |
French, Professor P |
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| Department: |
Physics |
| Organisation: |
Imperial College London |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 October 1997 |
Ends: |
31 December 1999 |
Value (£): |
209,001
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| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Fluorescence Lifetime Imaging (FLIM) is a powerful technique which can provide information about fluorophore environment where this impacts the fluorescence decay time. This is important for biological microscope and bioprocess monitoring applications where reporter fluorophores, which may be linked to particular cells, exhibit a fluorescence lifetime which is sensitive to e.g. pH, pO2, pCO2, glucose, [Ca2+] etc. FLIM is superior to intensity imaging for laser induced fluorescence measurements made in the presence of strong optical scattering, e.g. for in vivo biomedical applications. This proposal aims to develop a 100ps FLIM system which is desirable to detect environmental perturbations to fluorophore lifetimes. This will be employed in conjunction with an optical microscope to provide high temporal and spatial resolution at the cellular level. FLIM will also be investigated for application through biological tissue. The optimum spectral/temporal properties of the fluorophore dyes for in vivo imaging will be investigates - with emphasis on testing near infra-red fluorophore to take advantage of low absorption in biological tissue for this spectral region. The degradation of FLIM images by multiple scattering will be assessed and image reconstruction techniques (spatial filtering and inverse scattering calculations) will be investigated.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.imperial.ac.uk |