EPSRC logo

Details of Grant 

EPSRC Reference: GR/K96830/01
Title: DEVELOPMENT OF ADVANCED ANALYTICAL TOOLS FOR THE RAPID SCREENING OF IMMOBILISED COMBINATORIAL LIBRARIES
Principal Investigator: Tendler, Professor S
Other Investigators:
Davies, Professor M Roberts, Professor C
Researcher Co-Investigators:
Project Partners:
Department: Sch of Pharmaceutical Sciences
Organisation: University of Nottingham
Scheme: Standard Research (Pre-FEC)
Starts: 01 September 1996 Ends: 30 November 1998 Value (£): 156,308
EPSRC Research Topic Classifications:
Analytical Science
EPSRC Industrial Sector Classifications:
Pharmaceuticals and Biotechnology
Related Grants:
Panel History:  
Summary on Grant Application Form
Combinatorial libraries provide a novel solution to the problem of compound generation. The potential to immobilise libraries on to 2-D arrays, necessitates the development of analytical instrumentation to identify high affinity compounds. We propose to develop instrumentation which will allow the rapid location of potential lead compounds and their release for analysis. The project will exploit the ability of probe microscopes to measure sub-nanoNewton forces of interaction, with nanometre spatial precision. Forces of interaction between receptors immobilised on the probe and their ligands immobilised on substrates will be quantified in aqueous environments and mapped over a substrate to identify and quantify areas of chemical functionality. We have expertise in the design and construction of analytical instruments and the analysis of biomolecular interactions. For example a high resolution atomic force microscope (AFM) and a combined AFM/surface plasmon resonance instrument have been developed. We also have expertise and resources in sample preparation and biomolecular immobilisation. This application takes advantage of an agreement with Glaxo-Wellcome-Affymax for the supply of state-of-the-art combinatorial libraries arrays and a collaboration with Prof. Bycroft (University of Nottingham) who has expertise in the synthesis of combinatorial libraries.Key Words: Screening Analysis, Arrays, Local Probes, Molecular Forces, Sensors, Combinatorial Libraries, Nanotechnology, Biomolecular Immobilisation, Surfaces.
Key Findings
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
Potential use in non-academic contexts
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
Impacts
Description This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
Summary
Date Materialised
Sectors submitted by the Researcher
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
Project URL:  
Further Information:  
Organisation Website: http://www.nottingham.ac.uk