| EPSRC Reference: |
GR/A90206/01 |
| Title: |
SYNTHESIS, HYBRIDISATION AND ENZYMATIC MANIPULATION OF OLIGONUCLEOTIDES BONDED TO SILICON SURFACES |
| Principal Investigator: |
Houlton, Professor A |
| Other Investigators: |
|
| Researcher Co-Investigators: |
|
| Project Partners: |
|
| Department: |
School of Chemistry |
| Organisation: |
Newcastle University |
| Scheme: |
Advanced Fellowship (Pre-FEC) |
| Starts: |
01 October 1999 |
Ends: |
30 September 2004 |
Value (£): |
236,886
|
| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
|
|
| EPSRC Industrial Sector Classifications: |
| Electronics |
Pharmaceuticals and Biotechnology |
| No relevance to Underpinning Sectors |
|
|
| Related Grants: |
|
| Panel History: |
|
|
Summary on Grant Application Form |
|
It is proposed to use novel chemistry developed for the functionalisation of hydrogen-terminated silicon to synthesise short oligonucleotides at crystalline silicon surfaces. The ability of the resulting surface-tethered oligonucleotides to interact via hybridisation with complementary DNA strands will be explored. Finally, the ability of these surface-bound nucleic acids to act as substrates to enzyme systems which produce chemical transformations of DNA, such as nucleases and ligases, will be investigated. In this way chemical and biological syntheses are to be combined and exploited as a novel approach for the fabrication of semiconductor surfaces derivatized with biomolecules which may then be used to template further chemistry. This research would contribute to areas such as molecular electronics, biosensor design, and fundamental studies on DNA-mediated electron transfer.
|
|
Key Findings |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Potential use in non-academic contexts |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Impacts |
|
| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
|
| Date Materialised |
|
|
|
|
Sectors submitted by the Researcher |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
| Project URL: |
|
| Further Information: |
|
| Organisation Website: |
http://www.ncl.ac.uk |