| EPSRC Reference: |
GR/T02577/01 |
| Title: |
Small-Molecule Microarrays |
| Principal Investigator: |
Spring, Professor D |
| Other Investigators: |
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| Project Partners: |
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| Department: |
Chemistry |
| Organisation: |
University of Cambridge |
| Scheme: |
Faraday (PreFEC) |
| Starts: |
01 October 2004 |
Ends: |
30 September 2007 |
Value (£): |
178,744
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| EPSRC Research Topic Classifications: |
| Analytical Science |
Surfaces & Interfaces |
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| EPSRC Industrial Sector Classifications: |
| Pharmaceuticals and Biotechnology |
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Summary on Grant Application Form |
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The realistically-achievable programme for a three-year postdoctoral co-worker aims to validate the fabrication and exploitation small molecule microarrays (structured arrangement of small molecules such as pharmaceutical drugs in micron sized spots). Small molecule microarrays to date have suffered from low signal to noise ratios largely because they only have two-dimensional spot features, therefore we propose to use three-dimensional spot features to dramatically improve this. Small molecule microarrays will be fabricated and their properties assessed in order to optimise spot size, volume, assay compatibility and signal-to-noise ratio. The three dimensional microarrays will be used in (at least) three novel ways: 1) covalently attaching small molecules as probes (rather than DNA or proteins); 2) use gel pad microarrays as master templates and stamp replicates; and 3) use the hydrogel spots as spatially separated and addressable nano/pico litre 'wall-less test tubes'. In collaboration with two GSK CASE PhD students, a diversity-oriented and target-oriented library of different, discrete small molecules will be synthesised, all with appropriate functional groups compatible with covalent attachment to the small molecule microarrays. In collaboration with Genetix software will be developed for data handlinq and include intelligent feedback loos. The optimised small molecule microarrays will be exploited as an ultra hiqh throughput and low cost tool to identify molecular interactions between small molecules and any other molecular entity.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.cam.ac.uk |