| EPSRC Reference: |
GR/S64110/01 |
| Title: |
Microscopy for probing the orientation and re-orientation of flourescent markers |
| Principal Investigator: |
Neil, Professor MAA |
| Other Investigators: |
|
| Researcher Co-Investigators: |
|
| Project Partners: |
|
| Department: |
Physics |
| Organisation: |
Imperial College London |
| Scheme: |
First Grant Scheme Pre-FEC |
| Starts: |
30 June 2004 |
Ends: |
29 June 2007 |
Value (£): |
124,304
|
| EPSRC Research Topic Classifications: |
| Drug Formulation & Delivery |
Lasers & Optics |
|
| EPSRC Industrial Sector Classifications: |
| Electronics |
Pharmaceuticals and Biotechnology |
|
| Related Grants: |
|
| Panel History: |
|
|
Summary on Grant Application Form |
|
Fluorescent markers are an important tool in the visualisation of structures and processes at a microscopic level in biological systems. Often observed with a confocal or multiphoton microscope, the positional distribution of the markers is identified by the excitation and emission of flourescent light. However, both the excitation and emission processes can also reveal further information about the orientation and re-orientation of the fluorophores t the selective absorption and emmission of polarised light. The measurement of these processes can give further insight into the structure and mobility of labelled features within a cell. We proposed to construct a laser scanning microscope system which uses multi-photon excitation and time resolve confocal detection to investigate the selective absorption and the time evolution of the subsequent de-polarisation of the fluorescence emission. As fundamental feature of this proposal, we will investigate the use of novel excitation beam profiles with high numerical aperture lenses where we can control the polarisation of the exciting light in all 3 dimensions (axially as well as laterally).
|
|
Key Findings |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Potential use in non-academic contexts |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Impacts |
|
| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
|
| Date Materialised |
|
|
|
|
Sectors submitted by the Researcher |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
| Project URL: |
|
| Further Information: |
|
| Organisation Website: |
http://www.imperial.ac.uk |