| EPSRC Reference: |
GR/R05574/01 |
| Title: |
Macrocyclic Metal Complexes as a Part of Anti-Sense DNA Probe |
| Principal Investigator: |
Piletsky, Professor S |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Inst of Bioscience & Technology |
| Organisation: |
Cranfield University |
| Scheme: |
JREI |
| Starts: |
19 September 2001 |
Ends: |
18 September 2004 |
Value (£): |
109,868
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
Catalysis & Applied Catalysis |
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| EPSRC Industrial Sector Classifications: |
| Manufacturing |
Pharmaceuticals and Biotechnology |
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| Panel History: |
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Summary on Grant Application Form |
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The physical/chemical properties and applications of Macrocyclic metal complexes have been intensively studied. They act as catalysts for hydrolysis of phosphate diesters including those present in nucleic acids, but unfortunately often contaminate biological samples. We propose to investigate solid phase synthesis of these compounds as clean catalysts for use in hydrolysis of DNAs and RNAs. The specificity of the catalyst will be enhanced by incorporation of complementary sequences as anti-sense probes. Viral DNA of Human Parvovirus B19 virus will act as a model. Part of the complementary sequence of its viral core will be selected as an anti-sense probe. Nucleotide and macrocyclic metal complexes will be synthesised/coupled onto a solid phase support via linkers and will then the used to hydrolyse the parental DNA molecule. Challenges are development of suitable and effective coupling chemistry between the linker molecule, nucleotides and macrocyclic metal complexes, selection of bio-compatible solid matrices an optimising the efficiency of the catalysts. An HPLC-MS system specially requested for the project for chemical analysis and studying the efficiency and performance of the probes. The project will produce environmentally friendly biocatalysts for DNA and RNA and will lead to other practical applications including therapeutically drugs, solid phase extraction columns and use in biosensors.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.cranfield.ac.uk |