| EPSRC Reference: |
GR/M29030/01 |
| Title: |
HIGH-FIELD FOURIER TRANSFORM ION CYCLOTRON RESONANCE (FTICR) MASS SPECTROMETRY FOR THE UK ACADEMIC COMMUNITY |
| Principal Investigator: |
Derrick, Professor PJ |
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| Department: |
Chemistry |
| Organisation: |
University of Warwick |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 October 1998 |
Ends: |
31 January 2002 |
Value (£): |
262,840
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
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| EPSRC Industrial Sector Classifications: |
| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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The reliability of the ESI and nanospray modes of operation of the FTICR in positive-ion mode with buffered solutions is to be raised through modification of the ion sources and through improving the efficiency of ion transfer from source to trapped cell. Deuterium exchange is to be used both in the cell and in solution to probe non-covalent interactions, conformations and quaternary structures. Primary structures of principally biomolecules, but also clusters and polymers, are to be determined using tandem mass spectrometric techniques such as sustained off-resonance irradiation. The main method of ionization to be used is electrospray ionization (ESI) and its variant nanospray. Matrix-assisted laser desorption/ionization (MALDI) is to be developed to the point that it can be applied, principally to clusters and polymers.Collaborative projects in the area of structural biology are to be established and carried forward. Collaborative projects in polymer and cluster science are to be established and developed. Routine determination of molecular masses using ESI and nanospray is to be established and exploited for collaborative projects.- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Keywords describing areas of proposal.Fourier transform ion cyclotron resonance mass spectrometry; structural biology; polymer; cluster
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.warwick.ac.uk |