| EPSRC Reference: |
GR/J92644/01 |
| Title: |
DNA SEQUENCE ANALYSIS USING ELECTROROTATION AND TRAVELLING WAVE TECHNIQUES |
| Principal Investigator: |
Pethig, Professor R |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Sch of Electronics |
| Organisation: |
Bangor University |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 April 1994 |
Ends: |
30 June 1995 |
Value (£): |
23,785
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| EPSRC Research Topic Classifications: |
| Materials Characterisation |
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Summary on Grant Application Form |
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This collaborative project with Prof E M Southern of Oxford will combine poly- nucleotide sequence analysis and A.C. electrokinetic assay techniques for new approaches to the problem of DNA sequence analysis. An image analysis system will be developed for monitoring simultaneously the electrorotation rates of a large number of microbeads coated with a high surface concentration of octa-oligonucleotides of known sequences. Hybridisation of a DNA probe to an oligonucleotide will be detected as a change in the rate and sense of electrorotation. The use of travelling wave effects for the simultaneous monitoring of large numbers of beads will also be explored. Scaling up the procedure for the analysis of long DNA strands would be accomplished by assigning different oligonucleotide sequences to beads of different size, colour and fluorescent labelling, for example, and by increasing the number of electrode chambers used to impose the rotating electric fields or travelling waves.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.bangor.ac.uk |