| EPSRC Reference: |
GR/J26366/01 |
| Title: |
LIGHT AMPLI SWITCH IMAGE STORAGE ORDERED SURFACEBOUND ARRAYS LUCIFERASE MICODOTS PROTOTYPE BIOELECTRONIC DEV |
| Principal Investigator: |
Wharton, Professor C |
| Other Investigators: |
|
| Researcher Co-Investigators: |
|
| Project Partners: |
|
| Department: |
Biochemistry |
| Organisation: |
University of Birmingham |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 September 1993 |
Ends: |
31 August 1996 |
Value (£): |
197,108
|
| EPSRC Research Topic Classifications: |
| Materials Characterisation |
|
|
| EPSRC Industrial Sector Classifications: |
|
| Related Grants: |
|
| Panel History: |
|
|
Summary on Grant Application Form |
|
Our previous studies of the luciferase system entrapped in agarose gels will be extended to include enzyme 'dots', where the enzyme is immobilised on a small surface area. This will be achieved in collaboration with the Department of Electronics and Electrical Engineering, University of Glasgow, who have experience of the technology. The fundamental enzymic properties of surface-bound luciferase will be evaluated in a single dot device as a prelude to the investigation of possible uses of arrays of such dots. Surface attachment will allow improved spatial resolution for image storage since the 10 mm dots will be individually addressable, eg by optical fibres. Two colour photoactivation of enzyme dots will be achieved by caging both ATP and luciferin such that each responds to a different wavelength of light. This facility will be exploited in studies of pattern recognition using masks. By attachment of luciferase to electrode surfaces we will examine the effects of local electrochemical generation of oxygen and protons as modulators of enzyme activity as well as the effects of voltage and current. variable gain light amplification will be achieved by enzyme switching. Photolysis of glyceraldehyde phosphate dehydrogenase caged with a novel photolytic group will be used to generate ATP.
|
|
Key Findings |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Potential use in non-academic contexts |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Impacts |
|
| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
|
| Date Materialised |
|
|
|
|
Sectors submitted by the Researcher |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
| Project URL: |
|
| Further Information: |
|
| Organisation Website: |
http://www.bham.ac.uk |