Details of Grant 

EPSRC Reference:	EP/Z534389/1
Title:	" ECOEVOGENOME: How do genome defences and mobile genetic elements shape long-term bacterial genome evolution and adaptation?"
Principal Investigator:	Brockhurst, Professor MA
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Department:	School of Biological Sciences
Organisation:	University of Manchester, The
Scheme:	Frontier Res Guarantee TFS
Starts:	01 April 2025	Ends:	31 March 2030	Value (£):	2,176,202
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Summary on Grant Application Form
Two major conceptual breakthroughs have revolutionised our understanding of bacterial genome evolution during the past decade. First, genome re-sequencing of Lenski's long-term evolution experiment (LTEE) has revealed the complexity of mutational dynamics driving genome evolution, and that rates of molecular evolution are decoupled from rates of adaption through time, even for a model bacterium inhabiting the simplest of environments. Second, microbial genomics has revealed bacterial genomes to be dynamic battlefields replete with myriad defence systems (DSs) and menageries of mobile genetic elements (MGEs) that collaborate and compete with each other, whilst also spurring evolutionary innovation by driving the exchange of genetic material between lineages (horizontal gene transfer; HGT). These views of bacterial genome evolution are, however, disconnected because, by design, the LTEE used a model bacterium with a relatively simple genome depleted in MGEs and DSs compared to its counterparts in nature. Moreover, MGEs and DSs have only ever been included in short-term evolution experiments and never at levels of diversity seen in nature, meaning that their long-term impacts on bacterial genome evolution are unknown. To deliver a causal understanding of how MGEs and DSs shape long-term bacterial evolution requires that these conceptual breakthroughs be reconciled: augmenting the reductive complexity of LTEEs by integrating into this powerful approach the rich diversity of MGEs and DSs that we now know exists in nature. To achieve this, I will reboot the LTEE framework, using innovative experimental designs to directly test how MGEs and DSs shape the trajectory, tempo, and mode of long-term genome evolution and adaptation in bacteria. Discoveries from this project will transform our causal understanding of long-term bacterial evolution, enhancing our ability to predict the behaviour of natural systems, and forging an eco-evolutionary view of bacterial genomes.
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Organisation Website:	http://www.man.ac.uk