| EPSRC Reference: |
EP/M506862/1 |
| Title: |
Development and validation of a dynamic drug screening platform for ADME testing |
| Principal Investigator: |
Telling, Professor ND |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Inst for Science and Tech in Medicine |
| Organisation: |
Keele University |
| Scheme: |
Technology Programme |
| Starts: |
01 October 2014 |
Ends: |
31 March 2016 |
Value (£): |
46,020
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| EPSRC Research Topic Classifications: |
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| EPSRC Industrial Sector Classifications: |
| Pharmaceuticals and Biotechnology |
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| Related Grants: |
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| Panel History: |
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Summary on Grant Application Form |
The Caco-2 cell line is the gold standard for the prediction of drug absorption and permeability in vitro by mimicking the
small intestine. However, this model lacks the dynamic motion which represents one of the physiological functions of the
small intestine (peristalsis). In our research, we investigate the possible similarity between the static Caco-2 cell model and
the dynamic intestine by creating a dynamic in vitro environment using MICA technology. In order to obtain the dynamic
cells environment, MICA Biosystems' technology was applied to the Caco-2 cell line. With this technology it is possible to
interact, by the application of an external magnetic field, with the magnetic core nanoparticles specifically attached on the
cells membrane, and produce deformations on the membrane which mimic a dynamic cell in vivo environment e.g. in the
case of the gut similar to peristaltic contractions. Our results to date have shown that MICA' technology improves the
absorption of the selected drugs, and their permeability is more similar to that found in the human intestine. Our data
suggests that MICA' technology applied to Caco-2 drug permeability assay could be used to better predict, using in vitro
assays, the in vivo human drug absorption rates. This feasibility study will enable us to test rigorously against the gold
standards and generate a new assay for the pharmaceutical market.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.keele.ac.uk |