| EPSRC Reference: |
EP/M000192/1 |
| Title: |
Ultrafast time resolved protein X-ray crystallography |
| Principal Investigator: |
van Thor, Professor JJ |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Life Sciences |
| Organisation: |
Imperial College London |
| Scheme: |
Standard Research |
| Starts: |
05 January 2015 |
Ends: |
04 January 2019 |
Value (£): |
601,879
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| EPSRC Research Topic Classifications: |
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| EPSRC Industrial Sector Classifications: |
| No relevance to Underpinning Sectors |
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| Related Grants: |
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| Panel History: |
| Panel Date | Panel Name | Outcome |
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23 Jul 2014
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EPSRC Physical Sciences Chemistry - July 2014
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Announced
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Summary on Grant Application Form |
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We now have all the tools and the methodology that allows us to watch proteins function with structural resolution in real time with ultrafast time resolution, probing fundamental processes in biological activation. With the availability of femtosecond X-ray Free Electron Laser sources which now operate in the hard X-ray regime with femtosecond pulse duration, unique opportunities arise to gain fundamental new insight into ultrafast structural processes in proteins. Here, I propose a research programme on femtosecond time-resolved X-ray crystallography of light induced reactions in light sensitive protein crystals and supporting spectroscopic studies. The necessary preliminary work for such experiments are provided in the form of data collected at the Stanford Linac Coherent Light Source (LCLS), from ultrafast optical spectroscopy investigations of non-linear transitions and pulse shaping to control femtosecond population transfer performed in the home laboratory, and from supporting modeling of crystallographic data quality based on measured source parameters. In addition to the primary target, which is a femtosecond experiment with Photoactive Yellow Protein crystals, novel experiments are being developed firstly using picosecond synchrotron sources, to add to the existing choices for femtosecond pump-probe studies and focuses on photoswitchable fluorescent proteins.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.imperial.ac.uk |