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Details of Grant 

EPSRC Reference: EP/E01500X/1
Title: Volumetric and time-sequenced live-cell imaging
Principal Investigator: Greenaway, Professor A
Other Investigators:
Buller, Professor G Towers, Professor DP Stephens, Professor DJ
Warburton, Professor R Towers, Dr CE
Researcher Co-Investigators:
Project Partners:
Wellcome Trust
Department: Sch of Engineering and Physical Science
Organisation: Heriot-Watt University
Scheme: Standard Research
Starts: 03 July 2006 Ends: 02 January 2008 Value (£): 139,108
EPSRC Research Topic Classifications:
Cells Lasers & Optics
EPSRC Industrial Sector Classifications:
No relevance to Underpinning Sectors
Related Grants:
Panel History:  
Summary on Grant Application Form
This proposal aims to develop, customise and demonstrate a new technique for imaging dynamic 3-dimensional processes in living cells that may be either unstained or imaged using fluorescent markers. The technique proposed will achieve wide-field, 3-D imaging with a time-sequence capability, leading to a 3-D 'movies' of live cells that can be obtained with minimal photo-damage to those cells.Our current view of many processes occurring in cells is based on static, snapshot views of dead cells. Increasingly, cell-biologists with to study dynamic events occurring in living cells. Whatever imaging technique is used to obtain this information, the effects of the toxicity of stains and of photo-damage (which may be accentuated by the generation of free radicals during the photobleaching of flurophores) must be considered. Although techniques like confocal scanning microscopy are widely and successfully used, some experiments where photo-damage is a problem are still better performed using wide-field imaging techniques.This proposal is for the use of a passive 3-dimensional imaging technique based on the use of diffractive optical elements. This technique can provide flux-efficient (i.e. low damage) 'movies' of living cells using stains or flurophores, but also through 3-D phase-contrast imaging of unmodified cells. Through collaboration between Physicists and two live-cell imaging groups, we intend to demonstrate the techniques.
Key Findings
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Summary
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Further Information:  
Organisation Website: http://www.hw.ac.uk