| EPSRC Reference: |
EP/C516036/1 |
| Title: |
Micro-Spot Arrays For Label-Free DNA Analysis By Surface Enhanced Raman Scattering |
| Principal Investigator: |
Green, Professor M |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Electrical and Electronic Engineering |
| Organisation: |
Imperial College London |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 March 2005 |
Ends: |
28 February 2006 |
Value (£): |
58,954
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
Chemical Biology |
| Surfaces & Interfaces |
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| EPSRC Industrial Sector Classifications: |
| No relevance to Underpinning Sectors |
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| Panel History: |
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Summary on Grant Application Form |
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1). Fabricate a range of optimised substrates and demonstrate the presence or absence of aging effect upon performance and, in as much as possible, within a 12 month programme to look at the stability of substrates prepared earlier in the programme.(2). Prepare ranges of chemicals (oligo-nucleotides) with relevant anchor and linker to create a chemical array library and attach drops of specific probe molecules to local areas of a SERS substrate in a 2x2 matrix.(3). Explore ways to prevent the non-specific adsorption of analyte by covering non-probe surface Ag sites with inert molecules, e.g. mercaptopropane. {points (2) and (3) are linked}(4). Analyse the observed SERS spectra both before and after analyte presentation. Look for distance dependence effects by tests with different target species (e.g. ss and ds oligo-nucleotides with different chain lengths). Measure any variations on SERS arising from analyte presentation (e.g. pH and salt concentration).(5). Demonstration of spatial selectivity.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.imperial.ac.uk |