| EPSRC Reference: |
EP/C50982X/1 |
| Title: |
Membrane Enhanced Peptide Synthesis- A New Paradigm Peptide Synthesis Technology |
| Principal Investigator: |
Livingston, Professor A |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Chemical Engineering |
| Organisation: |
Imperial College London |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
28 March 2005 |
Ends: |
27 September 2008 |
Value (£): |
260,521
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| EPSRC Research Topic Classifications: |
| Chemical Synthetic Methodology |
Separation Processes |
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| EPSRC Industrial Sector Classifications: |
| Pharmaceuticals and Biotechnology |
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Summary on Grant Application Form |
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Peptides are a very important class of biological molecules which are made up of amino acids linked together in a chain. Currently, technology for synthesising peptides involves anchoring a first amino acid to an insoluble polymeric resin, and then attaching subsequent amino acids to this resin. The big advantage is that at the completion of each reaction adding a further amino acid, unreacted amino acid monomer can be cleaned away by washing the resin on a filter. However, this technology is difficult to scale up to large scale, and in the lab measuring amounts of resin, and keeping it well mixed, adds to complexity, and does not lend itself to easy monitoring of reactions.This research proposal seeks to take a completely new approach to small peptide synthesis. Reactions will be carried out in a homogeneous liquid phase, and at the conclusion of each reaction the solution will be washed on a solvent stable nanofiltration membrane that is able to retain the peptide of interest. Residual amino acid monomers and coupling agents will be washed through the membrane so they cannot interfere with subsequent steps. The initial amino acids present in the synthesiser will be made bigger to make it easier to separate them on the membrane. A Membrane Peptide Synthesiser will be constructed, and used to synthesise a peptide at least 5 amino acids in length.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.imperial.ac.uk |